[Reu_journal] June 14

[Tracy fanous]

This morning I practiced the injection and transvection procedure some more. 
  After lunch, John (the graduate assistant) showed me the fluorescent 
microscope.  We secured the tadpoles by placing them in a small groove 
carved into the bottom of a petri dish filled with water.  A weight 
constructed of a bent nail with fishing wire tightly strung across it served 
to anchor them into the groove so they would remain stationary.  After 
learning how to use the microscope and observing the fluorescence of the 
transfected cells, I practiced some more at extracting the brain from 
several specimens.  At the end of the day, Dr. Miskevich helped me begin the 
culture needed to prepare our DNA.  Once it was prepared, I placed it in the 
incubator for overnight culturing.

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